Journal: PLoS ONE

Article Title: Intravenous Treatment with a Long-Chain Omega-3 Lipid Emulsion Provides Neuroprotection in a Murine Model of Ischemic Stroke – A Pilot Study

doi: 10.1371/journal.pone.0167329

Figure Lengend Snippet: Sham-operated mice (Control) versus control group that received saline (Stroke) and treatment group that received OGV at stroke (a.s.). (A) COX-2-, (B) IL-6-, and (C) IL-10 protein levels, n = 8. Mean ± SEM, p*<0.05; p**<0.01; p***<0.001; One-Way ANOVA with Tukey post-test.

Article Snippet: Samples were incubated with primary antibodies [(Cox-2 (sc-1745), Santa Cruz Biotechnology, USA; IL-6 (PP012P2), IL10 (PP007P2), Acris Antibodies, USA] and corresponding secondary antibodies (#401515, #401353 and #401253, Calbiochem, Germany) conjugated to horseradish peroxidase and visualized by ECLplus TM Reagent (Amersham Biosciences, USA).

Techniques:

Journal: Gastroenterology Research and Practice

Article Title: Immune and Inflammatory Responses of the Intestinal Mucosa following Extended Liver Radiofrequency Ablation

doi: 10.1155/2017/3450635

Figure Lengend Snippet: Ileal tissue immunohistochemical expression of IL-6, TNF α , and NF κ B in rats subjected to either liver RFA (group RFA) or sham operation (group Sham). # p < 0.05, ∗ p < 0.001 versus group Sham.

Article Snippet: Slides were then incubated for 60 minutes with the mouse monoclonal antibody CD68 + (AbD Serotec, UK), the mouse monoclonal antibody CD4 + (Thermo Scientific Inc., Germany), the mouse monoclonal antibody CD8 + (Thermo Scientific Inc., Germany), the rabbit polyclonal antibody MAdCAM-1 (Biorbyt, UK), the rabbit polyclonal antibody IL-6 (Santa Cruz Biotechnology Inc., USA), the mouse monoclonal antibody TNF α (Acris Antibodies Inc., USA), or the mouse monoclonal antibody NF κ Β (Santa Cruz Biotechnology Inc., USA), or at 1 : 100, 1 : 150, 1 : 150, 1 : 250, 1 : 500, 1 : 500, or 1 : 500 dilutions, respectively.

Techniques: Immunohistochemical staining, Expressing

Journal: Journal of Advanced Veterinary and Animal Research

Article Title: Administration of neem ( Azadirachta indica A. Juss) leaf extract decreases TNF-α and IL-6 expressions in dextran sodium sulfate-induced colitis in rats

doi: 10.5455/javar.2020.g476

Figure Lengend Snippet: Immunohistochemical analysis of TNF-α results. From upper left to lower left slides in clockwise order showing category 0–3 of TNF- α expressions. (Magnification: 400x optical power).

Article Snippet: The specimens were incubated overnight at 4°C, then immune-stained with primary antibody (rabbit polyclonal IgG to bind the mice TNF-α and IL-6) (Wuhan Fine Biotech Co., Ltd., China) in a concentration of 1 mg/ml diluted by 1:600.

Techniques: Immunohistochemical staining

Journal: Journal of Advanced Veterinary and Animal Research

Article Title: Administration of neem ( Azadirachta indica A. Juss) leaf extract decreases TNF-α and IL-6 expressions in dextran sodium sulfate-induced colitis in rats

doi: 10.5455/javar.2020.g476

Figure Lengend Snippet: The effect of DSS induction on TNF-α and IL-6 expressions.

Article Snippet: The specimens were incubated overnight at 4°C, then immune-stained with primary antibody (rabbit polyclonal IgG to bind the mice TNF-α and IL-6) (Wuhan Fine Biotech Co., Ltd., China) in a concentration of 1 mg/ml diluted by 1:600.

Techniques: Control, Expressing

Journal: Journal of Advanced Veterinary and Animal Research

Article Title: Administration of neem ( Azadirachta indica A. Juss) leaf extract decreases TNF-α and IL-6 expressions in dextran sodium sulfate-induced colitis in rats

doi: 10.5455/javar.2020.g476

Figure Lengend Snippet: The effect of neem leaf extract on TNF-α expression and its comparison to mesalazine.

Article Snippet: The specimens were incubated overnight at 4°C, then immune-stained with primary antibody (rabbit polyclonal IgG to bind the mice TNF-α and IL-6) (Wuhan Fine Biotech Co., Ltd., China) in a concentration of 1 mg/ml diluted by 1:600.

Techniques: Expressing, Comparison

Journal: Frontiers in Pharmacology

Article Title: Effects of Inonotus obliquus on ameliorating podocyte injury in ORG mice through TNF pathway and prediction of active compounds

doi: 10.3389/fphar.2024.1426917

Figure Lengend Snippet: The expressions of renal TNF-α and IL-6 (×200). CON: the control group; ORG: the obesity-related glomerulopathy model; IO_L: the model group treated with Inonotus obliquus (75 mg/kg/day); IO_H: the model group treated with I . obliquus (150 mg/kg/day). Data were presented as mean ± SD. # p < 0.05 vs. the CON; * p < 0.05 vs. the ORG.

Article Snippet: After deparaffinization, antigen retrieval and blocking with goat serum, the section was incubating with anti-IL-6 or anti-TNF-α rabbit polyclonal antibodies (Servicebio, Wuhan, China) overnight at 4°C.

Techniques: Control

Journal: Frontiers in Pharmacology

Article Title: Effects of Inonotus obliquus on ameliorating podocyte injury in ORG mice through TNF pathway and prediction of active compounds

doi: 10.3389/fphar.2024.1426917

Figure Lengend Snippet: Molecular docking of IO against ORG based on active compounds and targets. (A) The overlapping compounds between IO against ORG and IO absorbed in blood. (B) Classification of active compounds of IO against ORG. (C) Structural formula of interpene F and trametenolic acid. (D) Molecular docking of interpene F and trametenolic acid with TNF-α and IL-6. IO: Inonotus obliquus ; ORG: obesity-related glomerulopathy.

Article Snippet: After deparaffinization, antigen retrieval and blocking with goat serum, the section was incubating with anti-IL-6 or anti-TNF-α rabbit polyclonal antibodies (Servicebio, Wuhan, China) overnight at 4°C.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Effects of Inonotus obliquus on ameliorating podocyte injury in ORG mice through TNF pathway and prediction of active compounds

doi: 10.3389/fphar.2024.1426917

Figure Lengend Snippet: Downstream regulatory mechanisms of TNF-α and IL-6. TNFR1: TNF-α receptor 1; TRAF1: TNF receptor associated factor 1; TRADD: TNFR1-associated death domain protein; MAPK: mitogen-activated protein kinase; IL-6R: Interleukin 6 receptor; JAK: Janus-activated kinase; STAT3: signal transducer and activator of transcription 3.

Article Snippet: After deparaffinization, antigen retrieval and blocking with goat serum, the section was incubating with anti-IL-6 or anti-TNF-α rabbit polyclonal antibodies (Servicebio, Wuhan, China) overnight at 4°C.

Techniques: